Sabinene synthase

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August undefined, 2024

WebAug 22, 2014 · A family of multiproduct (+)-3-carene synthase-like genes of Sitka spruce include the three (+)-3-carene synthases, PsTPS-3car1, PsTPS-3car2, PsTPS-3car3, and … WebDec 1, 2024 · In vitro enzyme assays showed that DcTPS54 is a single-product enzyme catalysing the formation of sabinene, whereas DcTPS04 is a multiple-product terpene synthase producing α-terpineol as a major ...

Monoterpene Synthases from Common Sage (Salvia officinalis ) *

WebMar 23, 2011 · The formation of terpene compounds is carried out by the terpene synthase (TPS) enzyme family, which generate various terpene products, including acyclic, monocyclic, and bicyclic terpenes, as well as terpene alcohols and diphosphate esters from various acyclic prenyl diphosphate precursors. Webthe MVA pathway, GPPS and sabinene synthase) and strain HB3 (E. coli harboring the native MEP pathway, GPPS syn-thase and sabinene synthase) were cultured to test the ef-fect of the MVA pathway on the production of sabinene, in fermentation medium under shake-flask conditions. The sabinene titer of strain HB4 reached 44.74 mg/L after being hdv altona

Microbial production of sabinene--a new terpene-based ... - PubMed

WebThe radio-GC profiles for the olefinic products generated from [1-3 H]geranyl diphosphate are illustrated for recombinant sage sabinene synthase (SSS), 1,8-cineole synthase (SCS), and bornyl diphosphate synthase (SBS), and for a crude enzyme extract from isolated sage oil gland secretory cells. WebIts essential oil mainly consists of linalool, α-pinene, terpinolene, β-pinene, myrcene, α-terpinene, sabinene, bornyl acetate, cadinene, carene, cedrol, and camphene that are of … WebDec 15, 2024 · Compartmentation of sabinene synthase in diverse combinations of subcellular location. (A) Analysis of subcellular location of peroxisomally and mitochondrially targeted sabinene synthase (t34SabS1). PEX3 and COX4 are the specific markers in peroxisomes and mitochondria, respectively. hdv haine

Characterization of a sabinene synthase gene from rough lemon …

The effects of water deficit on the expression of ... - Springer

WebDec 12, 2012 · There are three characterised sabinene hydrate synthases from plants [66, 67] which each make a mixture of (E)-and (Z)-sabinene hydrate, along with very small … WebSep 14, 2006 · Thus, Sf-CinS1 was successfully converted to a sabinene synthase with a minimum number of rationally predicted substitutions, while identification of the Asn side chain essential for water activation introduced 1,8-cineole and alpha-terpineol activity to Salvia pomifera sabinene synthase. A major contribution to product specificity in Sf-CinS1 … aterian 회사WebApr 24, 2024 · GPPS2, geranyl diphosphate from Abies grandis; SabS1, sabinene synthase from Salvia pomifera. b Schematic diagram of plasmids pHB7 and pTrcLower. Full size image. Most studies on the engineering of microbes to produce various chemicals by metabolic engineering or synthetic biology focused on improving the metabolic efficiency … aterian plc beta

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Sabinene synthase

Webthe particular presence of a ( )-sabinene synthase as a closely related enzyme with a group of ( )-3-carene synthases sug-gested a pattern of divergent evolution in which PsTPS-sab arose from a PsTPS-3car ancestor through gene duplication and shift of function (7). WebFeb 10, 2014 · Microbial production of sabinene--a new terpene-based precursor of advanced biofuel This is the first report of microbial synthesis of sabinene using an engineered E. coli strain with the renewable carbon source as feedstock. Therefore, a green and sustainable production strategy has been established for sabinene.

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WebNov 12, 2024 · The (+)-sabinene synthase (SS) is the first enzyme in the production of two major monoterpenes, alpha- and beta-thujone. The 1,8-cineole synthase (CS) catalyzes the one-step conversion of GPP to 1,8-cineole (Croteau and Karp 1977). WebMay 24, 2024 · For sabinene production the limonene synthase mutant N345A was used, which produces sabinene as its major product along with smaller amounts of other …

WebAug 22, 2014 · Plasticity and evolution of (+)-3-carene synthase and (-)-sabinene synthase functions of a sitka spruce monoterpene synthase gene family associated with weevil resistance Plasticity and evolution of (+)-3-carene synthase and (-)-sabinene synthase functions of a sitka spruce monoterpene synthase gene family associated with weevil … WebAnd demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar.RESULTS:In this study, sabinene was significantly produced by assembling a biosynthetic pathway using the methylerythritol 4-phosphate (MEP) or heterologous mevalonate (MVA) pathway combining the GPP and sabinene …

WebApr 14, 2024 · Above-ground material of members of the mint family is commercially distilled to extract essential oils, which are then formulated into a myriad of consumer products. Most of the research aimed at characterizing the processes involved in the formation of terpenoid oil constituents has focused on leaves. We now demonstrate, by … WebThe Sabinene synthase (ss) gene expression rate enhanced notably under drought stress and the highest expression rate was obtained in the 40% FC in plants inoculated with P. fluorescens. In...

WebFeb 1, 2024 · Sabinene is an important naturally occurring bicyclic monoterpene which can be used as flavorings, perfume additives, fine chemicals, and advanced biofuels. Up to …

WebAnd demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar.RESULTS:In this study, sabinene was significantly … aterian partnersWebJul 1, 1998 · gously expressed synthases produce ( 1 )-bornyl diphos- phate, 1,8-cineole, and ( 1 )-sabinene, respectively, as their major products from geranyl diphosphate. The bornyl diphosphate synthase... hdvision entertainmentWebsage sabinene synthase; SUS, sage unknown synthase. SCHEME 1. Conversion of geranyl diphosphate to the monoterpenes of sage. Formation of the monocyclic and bicyclic products requires preliminary isomerization of geranyl diphosphate to linalyl diphosphate. Acyclic products can be formed from either geranyl diphosphate or linalyl ateriankorvike patukkaWebJul 24, 2014 · HcTPS7 designated as sabinene synthase was a multiproduct enzyme that synthesizes sabinene (74.5 %) as its main product and nine by-products (α-thujene, α-pinene, β-pinene, myrcene, α-phellandrene, α-terpinene, … aterian yahoo hdvhdWebJun 12, 1998 · Monoterpene Synthases from Common Sage (Salvia officinalis ): cDNA ISOLATION, CHARACTERIZATION, AND FUNCTIONAL EXPRESSION OF (+)-SABINENE SYNTHASE, 1,8-CINEOLE SYNTHASE, AND (+)-BORNYL DIPHOSPHATE SYNTHASE - ScienceDirect Volume 273, Issue 24, 12 June 1998, Pages 14891-14899 … aterian muskariaWebAug 19, 2024 · Artemisia absinthium—wormwood (Asteraceae)—is a very important species in the history of medicine, formerly described in medieval Europe as “the most important master against all exhaustions”.It is a species known as a medicinal plant in Europe and also in West Asia and North America. The raw material obtained from this species is Absinthii … hdv kiel