WebCryo-EM, Part B: 3-D Reconstruction Yao Cong, Steven J. Ludtke, in Methods in Enzymology, 2010 6 CTF and Envelope Correction The contrast transfer function (CTF) … WebMay 1, 2024 · We present a new method for CTF estimation based on multitaper techniques that reduce bias and variance in the estimate. We also use known properties of the CTF …
Reducing bias and variance for CTF estimation in single ... - PubMed
WebNovaCTF is a freeware for 3D-CTF correction for electron microscopy, as described in this publication: Turoňová, B., Schur, F.K.M, Wan, W. and Briggs, J.A.G. Efficient 3D-CTF correction for cryo-electron tomography … WebJan 1, 2024 · goCTF: Geometrically optimized CTF determination for single-particle cryo-EM J Struct Biol. 2024 Jan 1;205(1):22-29. doi: 10.1016/j.jsb.2024.11.012. Epub 2024 Nov … diane wallis
Cryo-EM structures of mitochondrial ABC transporter …
WebIMOD Guides, Assistance and Information. The IMOD User's Guide with installation and other general information.; Tomography Resources. A Tomography Guide, with detailed instructions on building tomograms with the IMOD package.; A Tutorial for building a dual-axis tomogram with Etomo, and a sample data set.This tutorial now uses the unbinned … WebThis tutorial covers the entire single- particle analysis workflow in relion-3.0: beam-induced motion-correction, CTF estimation; automated particle picking; particle extraction; 2D class averaging; SGD-based initial model generation; 3D classification; high- resolution 3D refinement; CTF refinement and beamtilt estimation; the processing of movies … WebMay 10, 2024 · Surprisingly, after CTF refinement, the dataset reached a resolution very similar to the other datasets (a), which confirms the accuracy and robustness of aberration correction in Relion. (c) At first, set 3 (noZLF) reached a resolution that was much lower than the other datasets. ... Electron-event representation data enable efficient cryoEM ... citi aadvantage platinum business